Primary structure and functional expression of a glutaminyl cyclase.
收藏PubMed Central1991-11-15 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC52867/
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资源简介：
A glutaminyl cyclase (QC) that is probably involved in the biosynthesis of pyroglutamyl peptides such as gonadotropin-releasing hormone and thyrotropin-releasing hormone has been purified to homogeneity from bovine anterior pituitary. On the basis of N-terminal sequence analysis, a 2088-base-pair cDNA clone was isolated from a bovine anterior pituitary library. From the nucleotide sequence of this clone, the primary structure of a 330-residue protein and a preceding 31-residue prepropeptide sequence was deduced. By transfection of COS-7 monkey cells with a QC cDNA/pCDM8 vector construct, QC activity was expressed. Hybridization with mRNAs of various bovine tissues revealed expression of QC mainly in brain tissue. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1991-11-15
