Bacterial microbiota in nest samples - Experimental study

This study aim at testing the effect of aromatic plants on bacterial microbiota of nest samples in an experimental approach, and on bacterial microbiota of nest material and eggshell and on nestling traits and behavior in an observational approach in Corsican blue tits (Cyanistes caeruleus ogliastrae).The metadata and codes to analysis the data can be found in the following GitHub public repository: To add.<b>Experimental approach</b>We collected natural blue tit nests that have been predated or abandonned by the parents. We subdivised the nest material in nine subsamples that were placed in fake plastic nest boxes for 7 days. Every two days, we added fresh aromatic plant fragments on the top of the nest sample. The treatments corresponded to the addition of five single species (Lavandula stoechas, Mentha suaveolens insularis, Helichrysum italicum, Pulicaria odorata and Achillea ligustica), three combinations of plant duos frequently observed in blue tit nests (Lavandula stoechas and Mentha suaveolens insularis, Lavandula stoechas and Helichrysum italicum, and Mentha suaveolens insularis and Helichrysum italicum), and one control sample with no plant addition. At the end of the experiment, we collected the samples for the study of bacterial microbiota by extracting DNA and amplifing the 16S rARN sequences by PCR. We sequenced the samples with Illumina Mi-Seq.The dataset contains the bacterial forward and reverse reads of:<br>169 nest samples (9 treatments X 19 collected nests - 2 discarded samples)15 fresh plant samples (5 species X 3 samples)2 DNA extraction controls2 negative PCR controls2 positive PCR controls

本研究旨在通过实验方法探究芳香植物对科西嘉蓝山雀（*Cyanistes caeruleus ogliastrae*）巢样本的细菌菌群的影响，并通过观测方法分析其对巢材、蛋壳的细菌菌群以及雏鸟性状与行为的影响。本研究的元数据与数据分析代码可在以下GitHub公开仓库获取：待补充。<b>实验方法</b>我们采集了被天敌捕食或亲鸟弃巢的天然蓝山雀巢，将巢材划分为9份子样本，放置于仿真塑料巢箱中培养7天。每两日向巢样本顶部添加新鲜芳香植物片段。实验处理共设置5种单一植物物种（宽叶薰衣草*Lavandula stoechas*、海岛薄荷*Mentha suaveolens insularis*、意大利蜡菊*Helichrysum italicum*、臭蚤草*Pulicaria odorata*、意大利蓍*Achillea ligustica*）、3种蓝山雀巢中常见的双物种植物组合（宽叶薰衣草与海岛薄荷、宽叶薰衣草与意大利蜡菊、海岛薄荷与意大利蜡菊），以及1组不添加任何植物的空白对照组。实验结束后，我们通过DNA提取、聚合酶链式反应（PCR）扩增16S rRNA序列的方式采集样本以分析细菌菌群，并采用Illumina MiSeq平台完成测序。本数据集包含以下细菌正向与反向测序读段：<br>169份巢样本（9种处理×19个采集巢群-2份废弃样本）、15份新鲜植物样本（5个物种×3份重复样本）、2份DNA提取空白对照、2份PCR阴性对照、2份PCR阳性对照