Expression data from CSC and Non-CSC of three primary HCC samples

Hepatocellular carcinoma (HCC) represents the major subtype of liver cancer, characterized with a high rate of recurrence and heterogeneity. Liver cancer stem cells (CSCs) may account for a hierarchical organization of heterogeneous cancer cells. However, how liver CSCs sustain their self-renewal remains largely unknown. We used microarrays to discover the long non-coding RNAs (lncRNAs) expression underlying cell stem cell (CSC) and non cell stem cell (non-CSC) and identified distinct lncRNAs during this process. We sorted CD13+CD133+ and CD13-CD133- cells from three primary HCC patients as liver CSCs and non-CSCs, then hybridized on Aligent microarrays. We sought to identify distinct lncRNAs in liver CSCs.

肝细胞癌（Hepatocellular carcinoma, HCC）是肝癌的主要亚型，具有高复发率与显著异质性的生物学特征。肝癌干细胞（cancer stem cells, CSCs）被认为是构成异质性癌细胞层级组织结构的核心驱动因素。然而，肝癌干细胞如何维持其自我更新能力，目前仍尚未完全阐明。本研究通过基因芯片（microarrays）技术，筛选并鉴定了肝癌干细胞与非干细胞中差异表达的长链非编码RNA（long non-coding RNAs, lncRNAs）。我们从3名原发性肝细胞癌患者体内分选得到CD13阳性CD133阳性（CD13+CD133+）与CD13阴性CD133阴性（CD13-CD133-）细胞，分别作为肝癌干细胞与非干细胞，随后将其样本在Aligent基因芯片上完成杂交实验。本研究旨在鉴定肝癌干细胞中特异性表达的长链非编码RNA。