mRNA expression data from AG-haESC, E14 and MEF. Mus musculus

Haploid cells are amenable for genetic analysis because they contain only one set of chromosomes.Here,we report the derivation of haESCs from androgenetic blastocysts. These cells, which we designated AG-haESCs, express classical ESC markers, are pluripotent, and contribute to various tissues including the germline upon injection into diploid blastocysts. We used microarrays to compare the gene expression levels among androgenetic haploid embryonic stem cell lines(AG-haESC) E14 and male mouse embryonic fibroblasts (MEFs) and identified that most paternally imprinted genes were down-regulated and the maternally imprinted genes were up-regulated. Overall design: To avoid the influence of diploidized cells on the expression profile, we collected samples from FACS of cells at G1/G0 stage by staining Hochest 33342. We used E14,which was a male embryonic stem cell lines, and MEFs isloated from male individuals as control. Gene expression profiles of all the cell lines were analysed on an Affymetrix GeneChip 430 2.0 array.

单倍体细胞（Haploid cells）仅含一套染色体，因此适于开展遗传分析。本研究报道了从孤雄囊胚（androgenetic blastocysts）中分离得到单倍体胚胎干细胞（haploid embryonic stem cells, haESCs）的方法。我们将这类细胞命名为AG-haESCs，该类细胞表达经典的胚胎干细胞标志物，具备多能性；将其注射至二倍体囊胚后，可参与包括生殖系在内的多种组织的形成。
我们通过基因芯片技术比较了孤雄单倍体胚胎干细胞系AG-haESC E14与雄性小鼠胚胎成纤维细胞（MEFs）的基因表达水平，结果显示大多数父本印记基因呈下调表达，而母本印记基因呈上调表达。
实验整体设计：为避免二倍体化细胞对基因表达谱的干扰，我们通过Hochest 33342染色，利用荧光激活细胞分选术（Fluorescence Activated Cell Sorting, FACS）收集处于G1/G0期的细胞作为样本。本研究以雄性胚胎干细胞系E14以及从雄性个体中分离得到的MEFs作为对照，所有细胞系的基因表达谱均通过Affymetrix GeneChip 430 2.0芯片完成分析。