A comprehensive map of alternative polyadenylation in African American and European American lung cancer patients

We report the application of a novel method and analysis pipeline that sequences 3’end -enriched RNA to comprehensively map the APA landscape in lung cancer. The goals of this study are to identify the functional role of APA in determining transcriptomic heterogeneity in lung cancer and population differences in APA. Ninety-eight patients with histologically confirmed non-small cell lung cancer were selected from the National Cancer Institute-Maryland (NCI-MD) lung cancer study. The study includes 98 human tumor tissues and 98 adjacent non-involved tissues (n=196 total). AA = African American EA = European American

本研究报道了一种新型方法及分析流程的应用，该方法通过对3'端富集RNA进行测序，以全面绘制肺癌中可变多聚腺苷酸化（Alternative Polyadenylation，APA）的分布图谱。本研究旨在明确APA在肺癌转录组异质性形成中的功能作用，以及不同人群间APA的表达差异。本研究从美国国家癌症研究所-马里兰州分所（National Cancer Institute-Maryland，NCI-MD）肺癌研究队列中，筛选得到98例经组织学确诊的非小细胞肺癌患者。本研究共纳入98例肿瘤组织与98例癌旁正常组织，总样本量共计196例。其中，AA指非裔美国人（African American），EA指欧裔美国人（European American）。