GWAS and Coexpression Network Reveal Ionomic Variation in Cultivated Peanut

Peanut is an important legume providing products with nutrient dense including mineral nutrition. However, the genetic basis underlying the variations in the mineral composition in peanut is still unknown. Genome-wide association studies (GWAS) of the concentrations of 13 mineral elements combined with coexpression network were performed using a diverse panel of 120 genotypes mainly selected from the U.S. peanut mini core collection. A total of 36 significant quantitative trait loci (QTLs) associated with five elemental concentrations were identified with phenotypic variation explained (PVE) from 18.35% to 27.56%, in which 24 QTLs were for boron (B), 2 QTLs for copper (Cu), 6 QTLs for sodium (Na), 3 QTLs for sulfur (S), and 1 QTL for zinc (Zn). A total of 110 nonredundant candidate causal genes identified were significantly associated with elemental accumulation, which included one high-priority overlap (HPO) candidate gene arahy.KQD4NT, an important elemental/metal transporter gene located on LGB04 with position 5,413,913–5,417,353.花生是一种重要的豆科作物，其相关产品属于高营养密度品类，富含矿物质营养成分。然而，调控花生矿物质组成变异的遗传基础仍有待阐明。本研究以主要源自美国花生微型核心种质库的120份多样化基因型材料为研究群体，结合共表达网络分析，对13种矿质元素的浓度开展全基因组关联分析（Genome-wide association studies, GWAS）。本研究共鉴定出36个与5种矿质元素浓度显著相关的数量性状位点（quantitative trait loci, QTL），各位点的表型变异解释率（phenotypic variation explained, PVE）介于18.35%至27.56%之间；其中硼（B）相关QTL 24个、铜（Cu）相关QTL 2个、钠（Na）相关QTL 6个、硫（S）相关QTL 3个、锌（Zn）相关QTL 1个。研究共筛选得到110个与矿质元素积累显著相关的非冗余候选因果基因，其中包含1个高优先级重叠（high-priority overlap, HPO）候选基因arahy.KQD4NT；该基因属于重要的元素/金属转运蛋白基因，定位于LGB04染色体的5,413,913–5,417,353区间。