miRNA signature of young, replicative and TNF-α-induced premature senescent of human microvascular endothelial cells-lung (HMVEC-L)

Despite the fact that miRNAs have been extensively investigated for their involvement in diseases, senescence as well as inflammation, miRNAs involved in TNF-α- induced premature senescence remain to be uncovered. Hence, the present study aims to identify unique miRNAs and their respective signaling pathways in TNF-α-induced senescence in lung microvascular endothelial cells and novel targets for prevention or protection against premature senescence and endothelial hyperpermeability. We have employed Agilent Human MicroRNAs microarray platform to evaluate the expressions of 866 human miRNAs and 89 human viral miRNAs, based on Sanger miRNA database release 12.0. miRNA expression profiles were established for young, replicative and TNF-α-induced premature senescent of HMVEC-L.

尽管microRNA (miRNA)已被广泛研究其与疾病、衰老及炎症的关联，但参与肿瘤坏死因子α (TNF-α)诱导的过早衰老的miRNA仍有待探明。有鉴于此，本研究旨在鉴定肺微血管内皮细胞中TNF-α诱导衰老过程中独特的miRNA及其相应信号通路，并挖掘可用于预防或对抗过早衰老与内皮细胞高通透性的新型靶点。本研究采用安捷伦（Agilent）人类microRNA微阵列平台，基于Sanger miRNA数据库12.0版，对866个人类miRNA以及89个人类病毒miRNA的表达水平进行检测分析。我们成功构建了年轻、复制性衰老及TNF-α诱导过早衰老的HMVEC-L细胞的miRNA表达谱。