Supplemental Material for Bui and Li, 2019

Figures S1-S7 contains the following supplementary figures:
Figure S1 shows re-replication profiles for representative re-replicating strains containing the lys2InsEA14-CAN1 mutagenesis reporter cassette inserted at distinct chromosomal locations
Figure S2 shows basal and re-replication induced gene amplification rates for the strains analyzed in Fig. 2
Figure S3 shows basal and re-replication induced reversion rates for lys2InsEA10 inserted at Site 1
Figure S4 shows basal and re-replication induced gene amplification rates for the strains analyzed in Fig. 3
Figure S5 shows basal and re-replication induced mutations rates for lys2InsEA14 and CAN1 inserted at Site 1 in mismatch repair mutants
Figure S6 shows basal and re-replication induced gene amplification rates for mismatch repair mutant strains analyzed in Fig. 6 and Fig. S5
Figure S7 shows basal and re-replication induced gene amplification rates for REV3 and rev3∆ strains analyzed in Fig. 7

Tables S1-S21 contains the following supplementary tables:
Table S1 contains the list of oligonucleotides used in this study
Table S2 contains the list of plasmids used in this study
Table S3 contains the list of yeast strains used in this study
Table S4 contains the basal and galactose induced rates of Lys+ graphed in Fig. 2B
Table S5 contains the basal and galactose induced rates of Ura+ graphed in Fig. S2
Table S6 contains the basal and galactose induced rates of Lys+ graphed in Fig. S3
Table S7 contains the basal and galactose induced rates of CanR graphed in Fig. 2C
Table S8 contains the basal and galactose induced rates of Lys+ graphed in Fig. 3A (pol2-4)
Table S9 contains the basal and galactose induced rates of CanR graphed in Fig. 3B (pol2-4)
Table S10 contains the basal and galactose induced rates of Ura+ graphed in Fig. S4 (pol2-4)
Table S11 contains the basal and (galactose induced rates of Lys+Ura+)/(galactose induced rates of Ura+) graphed in Fig 4B (pol2-4)
Table S12 contains the sequence of can1R mutations identified in this study
Table S13 contains the basal and galactose induced rates of Lys+ graphed in Fig. 6 & Fig. S5A (msh2∆)
Table S14 contains the basal and galactose induced rates of Lys+ graphed in Fig. S5A (mlh1∆)
Table S15 contains the basal and galactose induced rates of Ura+ graphed in Fig. S6A (msh2∆)
Table S16 contains the basal and galactose induced rates of Ura+ graphed in Fig. S6B (mlh1∆)
Table S17 contains the basal and galactose induced rates of CanR graphed in Fig. S5B (msh2∆)
Table S18 contains the basal and galactose induced rates of CanR graphed in Fig. S5B (mlh1∆)
Table S19 contains the basal and galactose induced rates of Lys+ graphed in Fig. 7A (rev3∆)
Table S20 contains the basal and galactose induced rates of CanRg raphed in Fig. 7B (rev3∆)
Table S21 contains the basal and galactose induced rates of Ura+ graphed in Fig. S7 (rev3∆)

补充图S1~S7包含以下内容：
图S1展示了携带整合于不同染色体位点的lys2InsEA14-CAN1诱变报告盒的代表性再复制菌株的再复制谱图
图S2呈现了图2中分析菌株的基础水平及再复制诱导的基因扩增速率
图S3展示了整合于位点1的lys2InsEA10的基础水平及再复制诱导的回复突变率
图S4呈现了图3中分析菌株的基础水平及再复制诱导的基因扩增速率
图S5展示了错配修复缺陷菌株中整合于位点1的lys2InsEA14与CAN1的基础水平及再复制诱导的突变率
图S6呈现了图6及图S5中分析的错配修复缺陷菌株的基础水平及再复制诱导的基因扩增速率
图S7展示了图7中分析的REV3野生型与rev3Δ缺失菌株的基础水平及再复制诱导的基因扩增速率

补充表S1~S21包含以下内容：
表S1收录了本研究使用的寡核苷酸列表
表S2收录了本研究使用的质粒列表
表S3收录了本研究使用的酵母菌株列表
表S4包含了图2B中绘制的Lys+的基础水平及半乳糖诱导速率
表S5包含了图S2中绘制的Ura+的基础水平及半乳糖诱导速率
表S6包含了图S3中绘制的Lys+的基础水平及半乳糖诱导速率
表S7包含了图2C中绘制的CanR的基础水平及半乳糖诱导速率
表S8包含了图3A（pol2-4）中绘制的Lys+的基础水平及半乳糖诱导速率
表S9包含了图3B（pol2-4）中绘制的CanR的基础水平及半乳糖诱导速率
表S10包含了图S4（pol2-4）中绘制的Ura+的基础水平及半乳糖诱导速率
表S11包含了图4B（pol2-4）中绘制的基础水平及（半乳糖诱导的Lys+Ura+速率）/（半乳糖诱导的Ura+速率）
表S12收录了本研究中鉴定的can1R突变的序列
表S13包含了图6及图S5A（msh2Δ）中绘制的Lys+的基础水平及半乳糖诱导速率
表S14包含了图S5A（mlh1Δ）中绘制的Lys+的基础水平及半乳糖诱导速率
表S15包含了图S6A（msh2Δ）中绘制的Ura+的基础水平及半乳糖诱导速率
表S16包含了图S6B（mlh1Δ）中绘制的Ura+的基础水平及半乳糖诱导速率
表S17包含了图S5B（msh2Δ）中绘制的CanR的基础水平及半乳糖诱导速率
表S18包含了图S5B（mlh1Δ）中绘制的CanR的基础水平及半乳糖诱导速率
表S19包含了图7A（rev3Δ）中绘制的Lys+的基础水平及半乳糖诱导速率
表S20包含了图7B（rev3Δ）中绘制的CanR的基础水平及半乳糖诱导速率
表S21包含了图S7（rev3Δ）中绘制的Ura+的基础水平及半乳糖诱导速率