Centropyge interrupta Genome sequencing. Centropyge interrupta

Samples of Centropyge interrupta (voucher no. 367) were purchased from local aquarium and originally imported from Izu, Japan. Total genomic DNA was extracted from muscle tissue. Paired-end genomic library was constructed by using Illumina TruSeq DNA PCR-Free Library Preparation Kits and then performed next generation sequencing by Miseq splatform (2X300bp).

凭证标本号为367的中断刺尻鱼（Centropyge interrupta）样本购自当地水族商家，其最初引种自日本伊豆地区。从该样本的肌肉组织中提取总基因组DNA（total genomic DNA）。采用Illumina TruSeq DNA无PCR文库制备试剂盒构建双端基因组文库，随后通过Miseq测序平台（2×300bp）完成下一代测序。