Aqueous wheat extract (Triticuma estivum) prevents carboplatin-induced myelosuppression and oxidative stress in Wistar rats

ABSTRACT: The present study aimed to evaluate the use of aqueous wheat extracts as an adjunct to antineoplastic therapy with carboplatin. In this study, 32 rats were used which were randomly distributed into 4 groups: G1 - negative control; G2 - control treated with physiological solution; G3 - animals treated with aqueous extract of wheat in the concentration of 100mg/kg; G4 - animals treated with aqueous wheat extract at the concentration of 400mg/kg; 300mg/m² of carboplatin was administered intraperitoneally at day 0 in animals from groups G2, G3, and G4, whereas 1ml of physiological solution was administered by the same route in animals from group G1. Animals were treated daily for 21 days by orogastric gavage according to their respective experimental group. Blood was collected from animals on days 3, 7 and 21 for complete blood count (CBC), biochemistry, and measurement of paraoxonase 1 (PON1) activity. On day 21, animals were euthanized and necropsied. Promising results were obtained regarding oxidative balance in groups G3 and G4. Both presented better PON1 activity in comparison with group G2 (P

摘要：本研究旨在评估小麦水提物作为卡铂（carboplatin）抗肿瘤治疗辅助手段的应用效果。本研究共使用32只大鼠，将其随机分为4组：G1为阴性对照组；G2为生理盐水处理对照组；G3为给予100mg/kg浓度小麦水提物组；G4为给予400mg/kg浓度小麦水提物组。于第0天对G2、G3、G4组大鼠腹腔注射300mg/m²的卡铂，G1组大鼠则经相同途径给予1ml生理盐水。各组大鼠每日按照实验分组方案经口胃管给药，持续21天。分别于第3、7、21天采集大鼠血液，用于全血细胞计数（CBC）、生化检测以及对氧磷酶1（PON1）活性测定。于第21天对大鼠实施安乐死并进行尸检。G3与G4组在氧化平衡方面取得了颇具前景的结果，二者的PON1活性均优于G2组（P