Fluorescent Macrophages in Drosophila Embryo

This is a data set that contains a time sequence of<strong> fluorescently labelled macrophages</strong> that migrated in a<strong> drosophila embryo</strong>. The macrophages were visualised in the embryo by using the UAS/GAL4 system.We used the srpHemo-Gal4driver,which mediate the expression of genes downstream of a UASsequence specifically in macrophages to express the following UAS fluorescent probes: UAS-RedStinger for the nuclei and UAS-Clip-GFP for the microtubules. Details of the imaging and preparation have been published in: Evans, I.R.; Zanet, J.; Wood, W.; Stramer, B.M.<em> Live imaging of Drosophila melanogaster embryonic339hemocyte migrations.Journal of visualized experiments:</em> JoVE2010,36. Details on the segmentation, tracking and analysis of the macrophage migration have been published in: José Alonso Solís-Lemus, Besaiz J Sánchez-Sánchez, Stefania Marcotti, Mubarik Burki, Brian Stramer, Constantino Carlos Reyes-Aldasoro, <em>Comparative study of contact repulsion in control andmutant macrophages using a novel interaction detection</em>, Journal of Imaging, BioRxiv, https://doi.org/10.1101/2020.03.31.018267 and Solís-Lemus, J.A.; Stramer, B.; Slabaugh, G.; Reyes-Aldasoro, C.C. <em>Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab</em>.Journal of Imaging 2019,5