Genome annotation of Ipomoea cavalcantei

The Carajás morning glory (<i>Ipomoea cavalcantei </i>D.F. Austin) is a plant species endemic to ironstone outcrops in the eastern Amazon, Brazil. Here, we present a chromosome-level reference genome combining PacBio HiFi and HiC sequencing data for this species. Our high-quality assembly of 1.02 Gb consisted of 314 contigs anchored to 15 chromosomes, with a contig N50 of 15.9 Mb and a scaffold N50 of 63.1 Mb. Benchmarking Universal Single-Copy Orthologue (BUSCO) analysis demonstrated that the genome assembly achieved high completeness, with a score of 94.92%. We obtained 25,335 unique protein-coding loci of which 92.09% were functionally annotated to the Refseq database. Repetitive sequences constituted 73.96% of the total genome length. This near-T2T genome paves the way for investigations into the species’ evolutionary history, molecular mechanisms associated with adaptation to its specialized niche, and to subsidize conservation efforts aimed at preserving this unique component of the Amazonian biodiversity.

卡雅斯牵牛花（*Ipomoea cavalcantei* D.F. Austin）是巴西亚马逊东部地区铁岩露头特有的植物物种。本研究针对该物种，结合PacBio HiFi与HiC测序数据，构建了染色体级别的参考基因组。该高质量基因组组装总长1.02 Gb，包含314个锚定至15条染色体的重叠群（contig），重叠群N50值为15.9 Mb，支架（scaffold）N50值为63.1 Mb。通用单拷贝同源基因基准评测（Benchmarking Universal Single-Copy Orthologue, BUSCO）分析结果显示，该基因组组装的完整度极高，评分达94.92%。本研究共预测得到25335个独特的蛋白质编码基因座，其中92.09%的基因座已在RefSeq参考序列数据库中完成功能注释。重复序列占基因组总长度的73.96%。该近乎端粒到端粒（Telomere-to-Telomere, T2T）的基因组，为该物种的进化历史、适应特殊生境的分子机制相关研究铺平了道路，同时也可为保护亚马逊生物多样性中这一独特组分的保育工作提供支撑。