Gene-expression profiles of primary cultures of cortical neurons and astrocytes.. Mus musculus

We used microarrays to compare the global programme of gene expression in primary cultures of neurons and astrocytes. These data sets were compared to the expression profiles of other tissues, including pancreatic islets, in order to identify a specific neuroendocrine program in pancreatic islets. Overall design: Neurons and astrocytes were isolated from brain cortex and cultured in vitro for 7 and 20 days respectively. RNA was extracted and hybridized on Affymetrix microarrays. Two biological samples from neurons and astrocytes were analyzed.

我们采用基因芯片（microarrays）技术，对比神经元与星形胶质细胞原代培养物的全基因组基因表达谱。将该数据集与包括胰岛在内的其他组织的表达谱进行比对，旨在鉴定胰岛中特异性的神经内分泌表达程序。
整体实验设计：从大脑皮层分离神经元与星形胶质细胞，分别体外培养7天与20天。提取RNA后在Affymetrix基因芯片上进行杂交实验。共分析来源于神经元与星形胶质细胞的两份生物学样本。