Effect of NCOA4-depletion on the transcriptome profile of J774 mouse macrophage cells. Effect of NCOA4-depletion on the transcriptome profile of J774 mouse macrophage cells

To determine the physiological roles of NCOA4 and ferritinophagy in macrophages, NCOA4 was depleted via liposome-mediated siRNA delivery into cells. Cells were treated with siRNA for NCOA4 depletion and then the transcriptome profiles acquired from RNA-seq were compared with those of control (scramble siRNA-treated) cells. Overall design: Comparative gene expression profiling of control J774 cells and those deficient in NCOA4 (n = 3 biological replicates per group)

为明确核受体辅激活因子4（NCOA4）与铁蛋白自噬（ferritinophagy）在巨噬细胞中的生理功能，本研究通过脂质体介导的小干扰RNA（siRNA）转染法敲低细胞内的NCOA4。实验中，使用靶向NCOA4的siRNA处理细胞以实现基因表达沉默，随后将通过RNA测序（RNA-seq）获得的转录组图谱与对照组（转染无义序列siRNA的细胞）的转录组图谱进行对比。实验整体设计：对对照组J774细胞与NCOA4缺陷型J774细胞开展比较基因表达谱分析，每组设置3个生物学重复。