Angiogenic factor imbalance precedes complement deposition in the placenta of preeclamptic-like BPH/5 mice

BPH/5 mice are an inbred strain with “borderline hypertension” that spontaneously develops both maternal and fetal hallmarks of preeclampsia. RNA-Seq analysis of BPH/5 uterine implantation sites at embryonic day 7.5, the peak of decidualization, identifies differential expression of inflammatory response genes, including members of the complement family, compared to C57 controls. Overall design: RNA-Seq was performed on RNA isolated from E7.5 BPH/5 and C57 implantation sites (n=4).BPH/5小鼠是一类携带有临界高血压（borderline hypertension）的近交系，可自发出现子痫前期（preeclampsia）的母体与胎儿特征性表型。在蜕膜化（decidualization）的峰值时期——胚胎第7.5天（E7.5），对BPH/5小鼠的子宫着床位点进行RNA测序（RNA-Seq）分析，结果显示相较于C57对照组，其炎症反应基因（包括补体家族成员）存在显著差异表达。实验设计概述：从胚胎第7.5天的BPH/5与C57小鼠着床位点中分离RNA，开展RNA测序（每组n=4）。