Gene expression profile of primary human naive and memory CD4+ T lymphocytes.

Gene expression data of primary human naive and memory CD4+T lymphocytes purified from peripheral blood are generated to be analyzed in different ways such as for traditional searching of differentially expressed genes between the two cell subsets or in combination to in-silico data of microRNAs target prediction for microRNAs known to be characteristically expressed in the two cell subsets. Two cell subsets (cell types) FACS purified from peripheral blood of six samples/healthy donors (samples #3,5,6,7,026,065). Naive CD4+ T cells were extracted from all 6 samples and 6 biological replicas were obtained (3N, 5N, 6N, 7N, 026N, 065N), while memory CD4+T cells were extracted from 4 samples and 4 biological replicas were obtained (3m, 5m, 6m, 7m). Both naive and memory replicas from samples 3, 5, 6 and 7 were hybridized onto two beadsarrays each while those from samples 026 and 065 were hybridized on 1 beadsarray each (for a total of 18 beadsarrays used). For analysis purposes naive cells samples 3N, 5N, 6N and 7N can be considered paired with memory samples 3m, 5m, 6m and 7m respectively, since they are obtained from the same blood samples/healthy donors.

本数据集包含从外周血中纯化得到的原代人初始型与记忆型CD4+T淋巴细胞的基因表达数据，可通过多种路径开展分析：既可用于传统的两类细胞亚群间差异表达基因检索，也可与已知在这两类细胞亚群中特征性表达的微小RNA（microRNAs，miRNAs）的计算机模拟靶基因预测数据结合分析。本研究从6名健康供者的外周血中，通过荧光激活细胞分选（Fluorescence Activated Cell Sorting, FACS）纯化获得两类细胞亚群：初始型CD4+T淋巴细胞从全部6份样本中提取，共得到6个生物学重复样本（3N、5N、6N、7N、026N、065N）；记忆型CD4+T淋巴细胞则从其中4份样本中提取，获得4个生物学重复样本（3m、5m、6m、7m）。来自样本3、5、6、7的初始型与记忆型重复样本均分别在2张beadarray芯片上完成杂交，而来自样本026和065的重复样本则各在1张beadarray芯片上完成杂交，总计使用18张beadarray芯片。从分析视角而言，样本3N、5N、6N、7N对应的初始型细胞可分别与样本3m、5m、6m、7m对应的记忆型细胞视为配对样本，二者均取自同一名健康供者的外周血样本。