The effect of AICD is independent of other APP metabolites including Aβ.

A–B, Decreased cell proliferation in FeCγ25;APP−/− mice compared to APP−/− mice. Photomicrograph reveals a reduced number of BrdU+ cells in the SGZ of FeCγ25;APP−/− compared to APP−/− which is quantified in (B) throughout the entire rostro-caudal extent of hippocampus. C, There was also a decrease in the number of immature neurons (DCX+) in the FeCγ25;APP−/− mice compared to APP−/−. Insets are magnified on the right to reveal the decreased number of DCX+ cells and the resulting dendritic branches in comparable regions of the hippocampus. (D) Quantitative analysis of the total number of DCX+ cells throughout the entire rostro-caudal extent of the hippocampus in 3-month-old animals reveal a statistically significant decrease in the number of DCX+ cells in the SGZ of FeCγ25; APP−/− mice compared to APP−/−mice.* p<0.05 (Student's T-test). n = 3 for all.

A图：与APP−/−小鼠相比，FeCγ25;APP−/−小鼠的细胞增殖能力减弱。显微照片显示，FeCγ25;APP−/−小鼠海马齿状回颗粒细胞下层（Subgranular Zone, SGZ）中的溴脱氧尿苷阳性（Bromodeoxyuridine-positive, BrdU+）细胞数量减少，该定量结果于(B)图中通过覆盖海马全吻尾轴范围的分析得以验证。C图：与APP−/−小鼠相比，FeCγ25;APP−/−小鼠体内的未成熟神经元（双皮质素阳性，Doublecortin-positive, DCX+）数量同样降低。右侧的放大插图可清晰展示海马对应区域内DCX+细胞数量及其树突分支的减少情况。D图：对3月龄动物海马全吻尾轴范围内的DCX+细胞总数进行定量分析，结果表明：FeCγ25;APP−/−小鼠SGZ区域内的DCX+细胞数量较APP−/−小鼠显著下降，差异具有统计学意义（学生t检验，*p<0.05）。所有实验组的样本量均为n=3。