DataSheet1_In situ gelling hydrogel loaded with berberine liposome for the treatment of biofilm-infected wounds.PDF

Background: In recent years, the impact of bacterial biofilms on traumatic wounds and the means to combat them have become a major research topic in the field of medicine. The eradication of biofilms formed by bacterial infections in wounds has always been a huge challenge. Herein, we developed a hydrogel with the active ingredient berberine hydrochloride liposomes to disrupt the biofilm and thereby accelerate the healing of infected wounds in mice.
Methods: We determined the ability of berberine hydrochloride liposomes to eradicate the biofilm by means of studies such as crystalline violet staining, measuring the inhibition circle, and dilution coating plate method. Encouraged by the in vitro efficacy, we chose to coat the berberine hydrochloride liposomes on the Poloxamer range of in-situ thermosensitive hydrogels to allow fuller contact with the wound surface and sustained efficacy. Eventually, relevant pathological and immunological analyses were carried out on wound tissue from mice treated for 14 days.
Results: The final results show that the number of wound tissue biofilms decreases abruptly after treatment and that the various inflammatory factors in them are significantly reduced within a short period. In the meantime, the number of collagen fibers in the treated wound tissue, as well as the proteins involved in healing in the wound tissue, showed significant differences compared to the model group.
Conclusion: From the results, we found that berberine liposome gel can accelerate wound healing in Staphylococcus aureus infections by inhibiting the inflammatory response and promoting re-epithelialization as well as vascular regeneration. Our work exemplifies the efficacy of liposomal isolation of toxins. This innovative antimicrobial strategy opens up new perspectives for tackling drug resistance and fighting wound infections.

研究背景：近年来，细菌生物被膜（bacterial biofilms）对创伤创面的影响及其防治手段已成为医学领域的核心研究课题。清除创面细菌感染所形成的生物被膜始终是一项重大挑战。本研究中，我们开发了一款搭载盐酸小檗碱脂质体（berberine hydrochloride liposomes）活性成分的水凝胶，通过破坏细菌生物被膜以加速小鼠感染创面的愈合。
研究方法：本研究通过结晶紫染色、抑菌圈测定、稀释涂布平板法等实验手段，评估盐酸小檗碱脂质体清除生物被膜的能力。受体外实验疗效的启发，我们选择将盐酸小檗碱脂质体负载于泊洛沙姆（Poloxamer）系列原位温敏水凝胶中，以实现与创面的充分接触并维持持久疗效。最终，对给药14天的小鼠创面组织开展了相关病理学与免疫学分析。
研究结果：最终结果显示，给药后创面组织中的细菌生物被膜数量显著降低，创面组织内各类炎症因子在短时间内即出现明显下调。与此同时，与模型组相比，给药组创面组织的胶原纤维数量以及参与创面愈合的相关蛋白表达水平均存在显著差异。
研究结论：本研究结果表明，盐酸小檗碱脂质体水凝胶可通过抑制炎症反应、促进再上皮化与血管再生，加速金黄色葡萄球菌（Staphylococcus aureus）感染创面的愈合。本研究验证了脂质体隔离毒素的应用效能。这一创新性抗菌策略为应对耐药性问题与对抗创面感染提供了全新视角。