UV mutagenesis conjugated to high throughput screening as a tool to generate new phenotypic diversity in wine yeast

The current global changes, societal and climatic, strongly challenge the wine industry. Multiple methods are applied in the development of new strains for the industry, but many are based on the existing phenotypic and genetic diversities. UV mutagenesis, as an untargeted strategy, has been successfully used for years, with significant examples on wine. Here we developed and validated a UV-mutant generation strategy coupled with a high throughput screening in wine-like conditions. This strategy led to the production of a 502 mutant’s library for which concentrations of eight primary metabolites after fermentation were assessed. This data paper presents the resulting data.当前全球范围内的社会与气候变革正对葡萄酒产业构成严峻挑战。学界已开发多种用于该产业的新型菌株培育方法，但多数均基于现有的表型与遗传多样性。紫外线诱变（UV mutagenesis）作为一种非靶向策略，已成功应用多年，在葡萄酒领域已有诸多成熟案例。本研究开发并验证了一种结合类葡萄酒环境下高通量筛选（high throughput screening）的紫外线突变体构建策略。该策略成功构建了包含502个突变体的文库，并对其发酵后八种主要代谢物的浓度进行了测定。本文数据集文章即对上述实验所得数据进行展示。