Radiation-induced gene translation profiles reveal tumor type and cancer specific components. Homo sapiens

The goal of this study was to compare the radiation-induced gene translation profiles generated from human tumor cell lines that are treated with radiation. Keywords: stimulus or stress design Overall design: A panel of cell lines included 5 gliomas, 4 pancreatic carcinomas, 3 breast carcinomas and 2 non-small cell lung carcinomas. In addition, radiation-induced gene translation profiles were generated for 4 normal human cell lines: a skin fibroblast (BJ), 2 lung fibroblasts (MRC5, MRC9) and mammary epithelial (MEC). Specifically, cell lines were exposed to 2 Gy or sham irradiated, polysome-bound RNA was isolated 6h later and subjected to microarray analyses. Each cell line was evaluated in biological replicates.

本研究旨在对比经放射处理的人类肿瘤细胞系所产生的辐射诱导基因翻译谱（radiation-induced gene translation profiles）。
关键词：刺激/应激实验设计
实验整体设计：本次研究的细胞系面板包含5株胶质瘤细胞系、4株胰腺癌细胞系、3株乳腺癌细胞系及2株非小细胞肺癌细胞系。此外，本研究还为4株正常人类细胞系构建了辐射诱导基因翻译谱：1株皮肤成纤维细胞（BJ）、2株肺成纤维细胞（MRC5、MRC9）以及1株乳腺上皮细胞（MEC）。
具体实验流程如下：将各细胞系分为2戈瑞（Gy）辐射照射组与假照射对照组，于处理6小时后分离多聚核糖体结合RNA，并进行微阵列分析。所有细胞系均设置生物学重复以完成实验评估。