Supporting data for “Functional genome-wide CRISPR screen in human gastric organoids reveals drivers of anoikis resistance”

"Proliferation assay" includes the record of cell counts and doubling time of organoids assessed by proliferation assay. "ROCK inhibitor withdrawal assay" includes the record of cell viability assays in ROCK inhibitor withdrawal assays. "Spheroid formation assay" includes the calculations of fold changes in cell number assessed by spheroid formation assays. "Collagen assay" includes the record of cell viability assays in collagen assays. "RNA_log2TPM" describes gene expression levels of in-house clinical samples by RNA-seq (in Log2TPM). "Sanger sequencing files" includes raw Sanger sequencing files of DNA of organoids with gene knockouts. "Western blot raw images" includes raw images of western blots.

“增殖检测（Proliferation assay）”包含经增殖实验评估的类器官细胞计数及倍增时间记录。“ROCK抑制剂撤除检测（ROCK inhibitor withdrawal assay）”包含该实验中的细胞活力检测记录。“球状体形成检测（Spheroid formation assay）”包含经球状体形成实验评估的细胞数量倍数变化计算结果。“胶原检测（Collagen assay）”包含该实验中的细胞活力检测记录。“RNA_log2TPM”描述了通过RNA测序（RNA-seq）获取的本机构临床样本的基因表达水平，以Log2TPM为单位。“Sanger测序文件（Sanger sequencing files）”包含携带基因敲除的类器官DNA的原始Sanger测序文件。“蛋白质免疫印迹原始图像（Western blot raw images）”包含蛋白质免疫印迹实验的原始图像数据。