Identification and Characterization of a Novel Long Noncoding RNA that Regulates Osteogenesis in Diet-Induced Obesity Mice

Non-insulin-dependent diabetes mellitus adversely alters bone cell functions, causing decreased bone quality. Emerging evidence suggests that long noncoding RNAs (lncRNAs) participate in a vast repertoire of biological processes and play essential roles in gene expression and posttranscriptional processes. LncRNAs are also implicated in the pathogenesis of many human diseases. To investigate the functional link the specific lncRNA and elucidate the molecular mechanisms, we assessed the structure of the bones from diet-induced obese (DIO) mice and wild type (WT) mice screened mRNAs and lncRNAs, and functionally identified a specific lncRNA. 18-week old male DIO mice (12-week high fat diet) and 18-week old male WT mice were used for microarray assay and comparison of the gene characteristics Jake, Chen

非胰岛素依赖型糖尿病（Non-insulin-dependent diabetes mellitus）会对骨细胞功能造成不良影响，进而导致骨质量下降。越来越多的研究证据表明，长链非编码RNA（long noncoding RNAs，lncRNAs）参与了广泛的生物学过程，并在基因表达及转录后调控过程中发挥关键作用。此外，长链非编码RNA还与多种人类疾病的发病机制密切相关。为探究特定长链非编码RNA的功能关联并阐明其分子机制，本研究评估了饮食诱导肥胖（diet-induced obese，DIO）小鼠与野生型（wild type，WT）小鼠的骨骼结构，同时筛选了二者的mRNA及长链非编码RNA，并通过功能实验鉴定出一种特定的长链非编码RNA。本研究选用经12周高脂膳食造模的18周龄雄性DIO小鼠，以及18周龄雄性WT小鼠进行基因芯片检测（microarray assay）及基因特征比较。作者：Jake, Chen