Transcriptome analysis of pluripotent stem cells and fibroblasts.

Purpose: The purpose of this data is to compare the transcriptome of embryonic stem cell (ESCs), Induced pluripotent stem cell (iPSCs) and fibroblasts using RNA-seq. Methods: Total RNA was harvested by Trizol (Invitrogen) and RNA profiles of samples were generated by sequencing. Illumina Casava 1.8 software used for basecalling. Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence (using fastx_trimmer) then mapped to hg19 (genome build) whole genome using Tophat. This study provides a framework for the application of pluripotent stem cells RNA expression dynamics compare to fibroblasts profiling toward characterization. Total RNA extracted from 3 human cell line samples representing hESCs, hiPSCs and fibroblasts.

研究目的：本数据集旨在通过RNA测序（RNA Sequencing, RNA-seq）技术，比较胚胎干细胞（Embryonic Stem Cells, ESCs）、诱导多能干细胞（Induced Pluripotent Stem Cells, iPSCs）与成纤维细胞的转录组。实验方法：采用Invitrogen公司的Trizol试剂提取总RNA，通过测序生成样本的RNA表达谱。碱基识别（basecalling）使用Illumina Casava 1.8软件完成。对测序得到的reads进行接头序列修剪，并借助fastx_trimmer工具对低复杂度或低质量序列进行屏蔽处理，随后通过Tophat将处理后的reads比对至hg19全基因组组装版本。本研究为多能干细胞相较于成纤维细胞的RNA表达动态分析及相关特征表征提供了研究框架。本研究从3株人细胞系样本中提取总RNA，分别对应人类胚胎干细胞、人类诱导多能干细胞与成纤维细胞。