Developmental transcriptome of the nematode Rhabditophanes sp. kr3021

In this study, we have characterized various developmental stages of the free-living nematode Rhabditophanes sp. kr3021. To test the dauer hypothesis which states that the infective larval stage in parasitic nematodes evolved from the dauer stage in free-living nematodes, we compared transcriptomic profiles of the parasitic Strongloides nematodes with the RNA-seq data of the most closely related free-living species Rhabditophanes. We had previously generated developmental transcriptomes of Rhabditophanes sp. kr3021 which were submitted as ENA project ID PRJEB36286 (ERP119451), but some replicates of these previously sequenced transcriptomes showed a relatively high degree of variability. Therefore, we repeated these experiments in a more controlled setting with more replicates.

本研究对自由生活线虫（free-living nematode）Rhabditophanes sp. kr3021的多个发育阶段进行了系统表征。为验证持久态假说（dauer hypothesis）——该假说提出寄生线虫的感染性幼虫阶段起源于自由生活线虫的持久态（dauer）阶段——我们比较了寄生类Strongloides线虫的转录组谱（transcriptomic profiles）与亲缘关系最近的自由生活物种Rhabditophanes的RNA测序（RNA-seq）数据。我们此前已完成Rhabditophanes sp. kr3021发育转录组的构建，相关数据已提交至欧洲核苷酸档案馆（ENA），项目编号为PRJEB36286（ERP119451），但此前测序得到的这些转录组中，部分生物学重复的变异程度相对较高。因此，我们在更为严格的控制条件下增设更多生物学重复，重新开展了相关实验。