The foam cell-derived exosomes exacerbate ischemic white matter injuryvia transmitting metabolic defectsto microglia

HMC3 were treated with modified OGD accompanied with NC-Exos or AS-Exos. HMC3 cells were collected into TRIZOL to extract total RNA. Libraries were constructed using eukaryotic common transcriptome libraries, and sequencing was performed using Novaseq-PE150 platform.将HMC3细胞经修饰氧糖剥夺（OGD）处理，并辅以阴性对照外泌体（NC-Exos）或AS-Exos。收集HMC3细胞并置于TRIZOL试剂中以提取总RNA。采用真核生物通用转录组文库构建方法制备测序文库，并使用NovaSeq-PE150测序平台完成测序。