miRNA expression of whole hippocampus 24hr post-injury in rat. Rattus norvegicus

Traumatic brain injury dysregulates microRNA expression in the brain. We hypothesized that injury-induced epigenetic changes contribute to neurodegeneration and learning and memory deficits after TBI. These changes may provide a mechanistic explanation for neuropsychiatric comorbidities in TBI patients. Our objective is to compare and contrast the effects of several neuroprotective drugs (JM6, PMI-006 and E33-estrogen) on the TBI-induced changes in microRNA expression in the hippocampus, a region of the brain that is critical for learning and memory. We will also study if different neuroprotective drugs have similar effects on common microRNAs which may cooperatively regulate a common set of gene targets. Overall design: 3 biological samples each of Naïve, Sham control, TBI and TBI plus JM6, TI plus PMI-006, and TBI plus E33 rat hippocampi were obtained 24 hr post-sham injury or TBI, stored in RNA later and sent to GenUs Biosystems for microRNA microarray analysis. [Support/contributor] The Moody Project for Translational Traumatic Brain Injury Research

创伤性脑损伤（TBI）会导致大脑内微小RNA（microRNA）的表达失调。本研究假设，损伤诱导的表观遗传改变会促成创伤性脑损伤后发生的神经退行性变以及学习记忆功能障碍，这类改变或可为创伤性脑损伤患者的神经精神共病提供机制层面的解释。本研究的目标为：对比数种神经保护药物（JM6、PMI-006与E33-雌激素）对创伤性脑损伤诱导的海马体（大脑中与学习记忆功能密切相关的脑区）内微小RNA表达改变的影响；同时探究不同神经保护药物对共有微小RNA的作用是否一致——这类共有微小RNA可协同调控一组共同的基因靶标。
实验整体设计：在假手术或创伤性脑损伤造模后24小时，分别采集未处理组（Naïve）、假手术对照组（Sham control）、创伤性脑损伤组（TBI）、TBI+JM6组、TBI+PMI-006组以及TBI+E33-雌激素组大鼠的海马体组织，每组设置3个生物学重复样本；样本经RNA later保存后，送至GenUs Biosystems公司开展微小RNA芯片分析。
[资助/贡献方] 穆迪创伤性脑损伤转化研究项目（The Moody Project for Translational Traumatic Brain Injury Research）