Antibiotic mediated perturbation profile of postnatal mice cecal microbiota

AVNM cocktail was administered orally daily from postnatal day three mice up to postnatal 28 days. Control groups were administered with the same volume of PBS. Pups were sacrificed in between at P7D, P14D, P21D and P28D and cecal microbiota was profiled using 16S rRNA gene sequencing. The main study goal of this project is to evaluate whether antibiotic-mediated perturbation in early postnatal days could alter the barrier integrity and IECs population and thus gut development.

本研究每日对出生后第3日的小鼠经口给予AVNM合剂（AVNM cocktail），持续至出生后第28日。对照组给予等体积的磷酸盐缓冲液（PBS）。分别于出生后第7日（P7D）、第14日（P14D）、第21日（P21D）及第28日（P28D）处死幼鼠，采用16S rRNA基因测序技术对其盲肠微生物群进行表征分析。本项目的主要研究目标为评估出生早期抗生素介导的菌群扰动是否会改变肠道屏障完整性与肠上皮细胞（Intestinal Epithelial Cells, IECs）群体，进而影响肠道发育。