Defining transcription factor networks that govers SCC growth [ChIP_seq_mm10]

ChIP-seq analyses were perform to define the distribution of Pitx1, Sox2, Trp63 and Klf4 through the genome and define their interactions in SCC. Overall design: ChIP-seq sequencing was done on TPC issolated from tumors or cultures cells in un differentiated or differentiated conditions. ChIPs for shPitx1 experimentes were performed 48 hours after Pitx1 depletion in replicates

本研究通过染色质免疫共沉淀测序（ChIP-seq）分析，明确转录因子Pitx1、Sox2、Trp63与Klf4在全基因组中的分布特征，并解析其在鳞状细胞癌（SCC）中的相互作用关系。总体实验设计：以从肿瘤组织中分离的TPC细胞，或处于未分化/分化培养条件下的培养细胞为材料开展ChIP-seq测序。针对shPitx1实验的ChIP实验，均在Pitx1基因敲降48小时后进行，并设置生物学重复。