VEGF production by KU812 and HMC-1 cells exposed to Dengue virus.

KU812 and HMC-1 cells were inoculated with Dengue virus-2 (DV) or UV-irradiated Dengue virus-2 (UDV) in the presence of human Dengue-immune serum (HDIS, 1∶1000 final dilution) or normal human serum (NHS, 1∶1000 final dilution), and VEGF levels in culture supernatants were then examined 24 h later. Significant VEGF production was not observed when KU812 and HMC-1 cells were infected with DV alone (data not shown).
HDIS in KU812*p<0.01 (HDIS and C48/80 versus UDV or C3/36), NHS in KU812 *p<0.01 (C48/80 versus DV, UDV or C3/36), HDIS in HMC-1 *p<0.01 (DV versus UDV, C3/36 or C48/80), NHS in HMC-1 *p<0.01 (C48/80 versus DV, UDV, or C3/36).
a: C3/36 medium alone served as a negative control.
b: Activation of mast cells with C48/80 (300 µg/ml) was used as a positive control.

将KU812与HMC-1细胞置于人登革热免疫血清（human Dengue-immune serum, HDIS，最终稀释比例1∶1000）或正常人血清（normal human serum, NHS，最终稀释比例1∶1000）中，分别接种登革病毒2型（Dengue virus-2, DV）或紫外线灭活登革病毒2型（UV-irradiated Dengue virus-2, UDV），随后于感染后24小时检测细胞培养上清液中的血管内皮生长因子（vascular endothelial growth factor, VEGF）水平。仅以DV单独感染KU812与HMC-1细胞时，未观察到显著的VEGF生成（数据未展示）。
KU812细胞的HDIS组：*p<0.01（HDIS联合C48/80组对比UDV组或C3/36组）；KU812细胞的NHS组：*p<0.01（C48/80组对比DV、UDV或C3/36组）；HMC-1细胞的HDIS组：*p<0.01（DV组对比UDV、C3/36或C48/80组）；HMC-1细胞的NHS组：*p<0.01（C48/80组对比DV、UDV或C3/36组）。
a. 仅以C3/36培养基作为阴性对照。
b. 采用300 μg/ml的C48/80激活肥大细胞，以此作为阳性对照。