Functional characterization of a heat shock factor regulated enhancer at coronary artery disease associated locus that controls CALCRL expression in endothelial cells

We performed RNA-seq in TeloHAEC with samples where CALCRL was silenced using siRNA or enhancer-8 deleted using CRISPR-cas9 system to investigate the genes that are differentially expressed under CALCRL knockdown. Overall design: mRNA expression profiling of teloHAEC cells following si-CALCRL and enhancer-8 CRISPR-Cas9 deletion.

我们采用经两种方式处理的永生化人肺微血管内皮细胞（TeloHAEC）样本开展RNA测序（RNA-seq）：一组通过小干扰RNA（siRNA）沉默CALCRL基因，另一组利用CRISPR-Cas9系统敲除增强子-8（enhancer-8），以探究CALCRL敲低条件下的差异表达基因。实验整体设计：对经si-CALCRL处理及增强子-8 CRISPR-Cas9敲除的永生化人肺微血管内皮细胞进行mRNA表达谱分析。