Lnc-RNA expression pattern in ETV6/RUNX1-driven b-cell precursor acute lymphoblastic leukemia (BCP-ALL). Homo sapiens

Overwhelming evidence indicates that long non-coding RNAs have essential roles in tumorigenesis. Nevertheless, their expression and role in pediatric B-cell precursor acute lymphoblastic leukemia has not been extensively explored. Here, we conducted a comprehensive analysis of the long non-coding RNA transcriptome in ETV6/RUNX1 positive BCP-ALL, one of the most frequent subtypes of pediatric leukemia. An ETV6/RUNX1 expression signature was established, consisting of 596 lncRNAs (434 up and 162 down) using expression analysis of a series of primary patient samples. Subsequently, RNA sequencing from BCP-ALL cell lines and shRNA-mediated silencing of ETV6/RUNX1, illustrated that lnc-NKX2-3-1, lnc-TIMM21-5, lnc-ASTN1-1 and lnc-RTN4R-1 are bona fide ETV6/RUNX1 targets and could serve as novel biomarkers of this prevalent subtype of human leukemia. Overall design: Gene expression profiling of 64 BCP-ALL patient samples

大量确凿证据表明，长链非编码RNA（long non-coding RNAs）在肿瘤发生过程中发挥至关重要的作用。然而，此类RNA在儿童B细胞前体急性淋巴细胞白血病（B-cell precursor acute lymphoblastic leukemia，BCP-ALL）中的表达模式与生物学功能尚未得到广泛深入的探究。本研究针对儿童白血病最常见亚型之一的ETV6/RUNX1阳性BCP-ALL，开展了长链非编码RNA转录组的全面分析。通过对一系列原代患者样本的表达谱分析，我们构建了包含596个长链非编码RNA（其中434个表达上调、162个表达下调）的ETV6/RUNX1表达特征基因集。随后，通过对BCP-ALL细胞系进行RNA测序以及短发夹RNA（shRNA）介导的ETV6/RUNX1基因沉默实验，证实lnc-NKX2-3-1、lnc-TIMM21-5、lnc-ASTN1-1及lnc-RTN4R-1均为确凿的ETV6/RUNX1靶标长链非编码RNA，可作为该高发人类白血病亚型的新型生物标志物。研究整体设计：对64例BCP-ALL患者样本进行基因表达谱分析。