Expression data (HG-U133 Plus 2.0) from human monocyte-derived cell types (TolDC, M-MDSC, MoDC). Expression data (HG-U133 Plus 2.0) from human monocyte-derived cell types (TolDC, M-MDSC, MoDC)

A better understanding of the mechanisms that induce and drive human suppressive myeloid cells, such as myeloid-derived suppressor cells (MDSCs) and tolerogenic dendritic cells (TolDCs), stimulates therapeutic approaches that intent to restore the immune balance in patients. Here, we compared gene profiles of in vitro generated M-MDSCs to TolDCs in the context of monocytes and monocyte-derived DCs (MoDCs). Overall design: Human M-MDSCs, TolDCs and MoDCs were generated in vitro from CD14+ purified Monocytes for 5 donors. Four cell types from 5 donors were used for RNA extraction and hybridization on Affymetrix microarrays. Three Monocyte samples failed QC leaving a total of 17 samples in this analysis.

深入阐明诱导并维持人类抑制性髓系细胞——如髓系来源抑制细胞（myeloid-derived suppressor cells, MDSCs）与耐受性树突状细胞（tolerogenic dendritic cells, TolDCs）——的调控机制，可为开发旨在恢复患者免疫稳态的治疗策略提供理论支撑。本研究针对单核细胞及单核细胞来源树突状细胞（monocyte-derived DCs, MoDCs）背景下体外诱导生成的M-MDSCs与TolDCs的基因表达谱，开展对比分析。实验设计：本研究从5名供者的CD14阳性纯化单核细胞中，体外诱导获得人源M-MDSCs、TolDCs及MoDCs。采集5名供者的4类细胞样本进行RNA提取，并在Affymetrix基因芯片上完成杂交检测。其中3份单核细胞样本未通过质量控制（quality control, QC），本次分析最终共纳入17份样本。