Bacterial dynamics of the plastisphere microbiome exposed to sub-lethal antibiotic pollution

Here, we describe the bacterial dynamics underlying this response in surface water bacteria at the community, resistome and mobilome level using a combination of methods (Next Generation Sequencing and qPCR), sequencing targets (16S rRNA gene, pre-clinical and clinical class 1 integron cassettes and metagenomes), technologies (short and long read sequencing) and assembly approaches (non-assembled reads, genome assembly and plasmid assembly).

本研究结合多种研究方法（下一代测序（Next Generation Sequencing）与定量PCR（qPCR））、测序靶点（16S核糖体RNA基因（16S rRNA gene）、临床前与临床1类整合子盒以及宏基因组）、测序技术（短读长测序与长读长测序）及序列组装策略（非组装读段（non-assembled reads）、基因组组装（genome assembly）与质粒组装（plasmid assembly）），阐述了地表水细菌在群落、耐药组（resistome）与移动遗传元件组（mobilome）层面上支撑该响应过程的细菌动态变化规律。