CIL:37049, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library

Specifications of the custom built two-photon microscope, including the laser sources, signal amplification, PMT specifications, and YFP filter sets are described in:
Ruthazer ES, Li J, Cline HT. 2006. Stabilization of axon branch dynamics by synaptic maturation. J Neurosci 26(13):3594-3603.

The methods of cell transfection with electroporation can be found here:
Bestman JE, Ewald RC, Chiu S-L, Cline HT. 2006. In vivo single-cell electroporation for transfer of DNA and macromolecules. Nat Protocols 1(3):1267-1272.

Further explanation can be found here:
Bestman JE, Cline HT. 2008. The RNA binding protein CPEB regulates dendrite morphogenesis and neuronal circuit assembly in vivo. Proc Natl Acad Sci U S A 105(51):20494-20499.

Bestman JE, Cline HT. 2009. The Relationship between Dendritic Branch Dynamics and CPEB-Labeled RNP Granules Captured in Vivo. Front Neural Circuits 3:10.

本定制化双光子显微镜的技术参数，包括激光光源、信号放大系统、光电倍增管（Photomultiplier Tube，PMT）规格及黄色荧光蛋白（YFP）滤光片组，详见以下文献：
Ruthazer ES, Li J, Cline HT. 2006. 《通过突触成熟稳定轴突分支动力学》. 《神经科学杂志》（J Neurosci）26(13):3594-3603.

细胞电穿孔转染方法可参阅以下文献：
Bestman JE, Ewald RC, Chiu S-L, Cline HT. 2006. 《用于DNA与大分子转染的活体细胞单电穿孔技术》. 《自然实验指南》（Nat Protocols）1(3):1267-1272.

更多相关说明可参考以下文献：
Bestman JE, Cline HT. 2008. 《RNA结合蛋白CPEB在活体内调控树突形态发生与神经元环路组装》. 《美国国家科学院院刊》（Proc Natl Acad Sci U S A）105(51):20494-20499.
Bestman JE, Cline HT. 2009. 《活体内捕获的树突分支动力学与CPEB标记的核糖核蛋白颗粒之间的关联》. 《神经环路前沿》（Front Neural Circuits）3:10.