Attempts for deriving extended pluripotent stem cells from common marmoset embryonic stem cells. Attempts for deriving extended pluripotent stem cells from common marmoset embryonic stem cells

Extended pluripotent stem cells (EPSCs) derived from mice and humans showed an enhanced potential for chimeric formation. By exploiting transcriptomic techniques, we assessed the differences in gene expression profile between extended EPSCs derived from mice and humans, and those newly derived from the common marmoset (marmoset; Callithrix jacchus). Although the marmoset EPSC-like cells displayed a unique colony morphology distinct from murine and human EPSCs, they displayed a pluripotent state akin to embryonic stem cells (ESCs), as confirmed by gene expression and immunocytochemical analyses of pluripotency markers and three-germ-layer differentiation assay. Importantly, the marmoset EPSC-like cells showed interspecies chimeric contribution to mouse embryos, such as E6.5 blastocysts in vitro and E8.5 epiblasts in vivo in mouse development. Also, we discovered that the perturbation of gene expression of the marmoset EPSC-like cells from the original ESCs resembled that of human EPSCs. Thus, we established the efficacy of the method for the derivation of marmoset EPSCs Overall design: RNA seq of marmoset EPSC-like cells

源自小鼠与人类的扩展多能干细胞（extended pluripotent stem cells，EPSCs）具备更强的嵌合形成潜能。本研究借助转录组学技术，对比分析了小鼠、人类来源的扩展多能干细胞，与新近诱导获得的普通狨猴（common marmoset，狨猴；Callithrix jacchus）来源的扩展多能干细胞之间的基因表达谱差异。尽管狨猴类扩展多能干细胞呈现出与小鼠、人类扩展多能干细胞截然不同的独特集落形态，但经多能性标志物基因表达检测、免疫细胞化学分析以及三胚层分化实验验证，其多能状态与胚胎干细胞（embryonic stem cells，ESCs）高度相似。尤为关键的是，狨猴类扩展多能干细胞可对小鼠胚胎产生种间嵌合贡献，例如体外培养的小鼠E6.5囊胚，以及小鼠体内发育的E8.5上胚层。此外，本研究发现，狨猴类扩展多能干细胞相较于其原始胚胎干细胞的基因表达扰动特征，与人类扩展多能干细胞的扰动特征高度相似。综上，本研究验证了诱导获得狨猴扩展多能干细胞的方法有效性。实验整体设计：狨猴类扩展多能干细胞的RNA测序。