Ileal expression data of mice fed with diet containing protein from various sources. Mus musculus

This study was designed to address key questions concerning the use of alternative protein sources for animal feeds and addresses aspects such as their nutrient composition and impact on gut function, the immune system and systemic physiology. We used casein (CAS), partially delactosed whey powder (DWP), spray dried porcine plasma (SDPP), soybean meal (SBM), wheat gluten meal (WGM) and yellow meal worm (YMW) as protein sources. We investigated the effects of feeding mice during a period of four weeks on semi-synthetic diets containing 30% of six different protein sources. Microarrays were used to detail the global gene expression in the ileal mucosae of mice. Overall design: Male C57BL/6J mice were stratified according to bodyweight and litter of origin into six dietary treatment groups. These mice were fed for four weeks with semi-synthetic diets containing one of the following protein sources. At the end of four weeks, mice were sacrificed by euthenesia and ileal tissue samples (scrapings) were collected for RNA extraction and hybridization on Affymetrix microarrays. Soybean meal (SBM) diet group served as reference to make comparisons with other experimental diets.

本研究旨在解决动物饲料替代蛋白源应用领域的核心问题，涵盖其营养组成、对肠道功能、免疫系统及全身生理状态的影响等多个方面。本研究选用酪蛋白（casein, CAS）、部分脱乳清粉（delactosed whey powder, DWP）、喷雾干燥猪血浆（spray dried porcine plasma, SDPP）、豆粕（soybean meal, SBM）、小麦谷朊粉（wheat gluten meal, WGM）以及黄粉虫（yellow meal worm, YMW）作为实验蛋白源。我们探究了为期四周的饲喂实验：给小鼠喂食含六种不同蛋白源（各占日粮30%比例）的半合成日粮，分析该处理对小鼠产生的影响。采用微阵列技术对小鼠回肠黏膜的全基因表达谱进行详细解析。总体实验设计：将雄性C57BL/6J小鼠按体重及出生窝别进行分层，划分为六个日粮处理组。小鼠连续四周饲喂含下述某一种蛋白源的半合成日粮。饲喂周期结束后，通过安乐死处死小鼠，采集回肠黏膜刮取物样本，用于RNA提取及Affymetrix微阵列杂交实验。本研究以豆粕（SBM）日粮组作为参照组，用于与其余实验组开展对比分析。