Investigating the impact of cigarette smoke on pulmonary cellular immune responses to influenza virus at the single cell resolution in mice.. Investigating the impact of cigarette smoke on pulmonary cellular immune responses to influenza virus at the single cell resolution in mice.

Single cell RNA-sequencing (scRNAseq) of lung immune cells from mice exposed to room air or cigarette smoke, infected with influenza A virus. Room air saline controls are also included. This analysis facilitates a comparison of cigarette smoke-associated changes to the pulmonary immune environment at the level of individual leukocytes and stromal cells. Overall design: BALB/c mice were exposed to room air or cigarette smoke for two weeks, and subsequently infected with 50 PFU of influenza A/FM/1/47-MA (H1N1). Control room air-exposed mice administered saline were also included. Smoke exposure was re-initiated from 3-7dpi, and whole lung samples were sampled and sequenced at 7dpi. n=4-5 mice were pooled per group. Reads were aligned to mouse genome reference and A/FM/1/47-MA cRNAs (details below).

本数据集包含暴露于室内空气或香烟烟雾、并感染甲型流感病毒的小鼠肺部免疫细胞的单细胞RNA测序（single cell RNA-sequencing, scRNAseq）数据，同时纳入室内空气+生理盐水对照组样本。本分析可在单个白细胞与基质细胞层面，对比香烟烟雾相关的肺部免疫微环境变化。实验设计详情如下：将BALB/c小鼠暴露于室内空气或香烟烟雾两周后，以50噬斑形成单位（PFU）感染甲型流感病毒A/FM/1/47-MA（H1N1）；同时设置仅暴露于室内空气并经生理盐水处理的对照小鼠。烟雾暴露于感染后第3至7天重新启动，并于感染后第7天采集全肺样本进行测序。每组合并4-5只小鼠的样本。测序读段将比对至小鼠参考基因组与A/FM/1/47-MA的互补RNA（cRNA）序列（详细信息见下文）。