Comparison of HBO1 siRNA-treated Human Umbilical Vein Endothelial Cells (HUVECs) and control siRNA-treated HUVECs. Homo sapiens

To determine the role of HBO1 in EC physiology, gene expression analysis was conducted on control and HBO1 siRNA-treated HUVECs. A total of 263 differentially regulated protein-coding transcripts were detected, many of which are key for growth and angiogenesis. Additionally, many genes involved in cell cycle, cell division, and DNA replication were dysregulated. HBO1-regulated genes were verified by qRT-PCR, including those with roles in vessel tone regulation (e.g. ACE1), vessel formation (e.g. CXCL16), EC activation (e.g. E-selectin), and cholesterol efflux (e.g. ABCG1). Overall design: HUVECs were treated with control siRNA or HBO1 siRNA for 72 hours. Subsequently, these samples were subjected to gene expression analysis using a custom agilent microarray that recognizes 26109 protein-coding transcripts and 30586 lncRNAs.

为明确组蛋白乙酰转移酶HBO1在内皮细胞（Endothelial Cell, EC）生理过程中的作用，本研究对阴性对照小干扰RNA（small interfering RNA, siRNA）处理组及HBO1 siRNA处理的人脐静脉内皮细胞（human umbilical vein endothelial cells, HUVECs）开展基因表达分析。共检测到263个差异表达的蛋白编码转录本，其中诸多转录本对细胞增殖与血管生成至关重要。此外，大量参与细胞周期、细胞分裂与DNA复制的基因出现表达失调。
研究通过实时荧光定量聚合酶链式反应（quantitative real-time polymerase chain reaction, qRT-PCR）验证了HBO1调控的基因，这些基因涵盖血管张力调节（如ACE1）、血管形成（如CXCL16）、内皮细胞激活（如E-选择素，E-selectin）以及胆固醇外流（如ABCG1）等多个功能类别。
实验整体设计：将HUVECs分别用阴性对照siRNA或HBO1 siRNA处理72小时，随后采用可识别26109个蛋白编码转录本与30586个长链非编码RNA（long non-coding RNA, lncRNA）的定制安捷伦（Agilent）基因芯片开展基因表达分析。