Fed State Prior to Hemorrhagic Shock and Polytrauma in a Porcine Model Results in Altered Liver Transcriptomic Response. Sus scrofa

We sequenced liver mRNA from 23 individual pigs (5 prefed and 18 fasted) taken at 4 separate time points to evaluate the change in gene expression over the course of hemorrhagic shock and resuscitation in response to a carbohydrate prefed state. Overall design: Examination of mRNA levels in liver biopsies from pigs at 4 timepoints throughout hemorrhagic shock and resuscitation

我们对23头个体猪的肝脏信使RNA（messenger RNA，mRNA）进行了测序，其中5头为碳水化合物预喂养组，18头为禁食组，共设置4个独立采样时间点，以探究失血性休克与复苏进程中，基因表达的变化及其对碳水化合物预喂养状态的响应。
实验整体设计：对失血性休克及复苏全过程中4个时间点采集的猪肝脏活检组织的mRNA水平开展检测。