The use of infection-niche mimicking media has minor impacts on meropenem resistance evolution in Pseudomonas aeruginosa

Five isolates from the endpoint population of each condition (HLM/lung + meropenem, TSB + meropenem, TSB antibiotic-free control and HLM/lung antibiotic-free control) along with the ancestral PAO1 strain were sequenced using Illumina short-read sequencing, with a target depth of coverage at 60X. DNA extraction, library preparation, and sequencing were performed by MicrobesNG (Birmingham, UK) according to their protocols, using the Illumina NovaSeq600 platform with a 250 bp paired-end protocol.针对每种实验条件（HLM/肺+美罗培南、胰蛋白胨大豆肉汤（Tryptic Soy Broth，TSB）+美罗培南、无抗生素TSB对照组以及无抗生素HLM/肺对照组）的终点种群各选取5株分离株，连同祖先菌株PAO1，采用Illumina短读长测序技术进行测序，目标覆盖深度设定为60×。本次实验的DNA提取、文库制备及测序工作均由英国伯明翰的MicrobesNG公司按照其标准操作流程完成，测序采用Illumina NovaSeq600平台及250 bp双端测序方案。