miRNA profiles in obese and normal weight periodontitis patients. Homo sapiens

Affymetrix GeneChip miRNA 3.0 microarrays were compared in gingival tissue biopsy samples from obese and normal weight patients with periodontitis Overall design: Total RNA was extracted from gingival tissue biopsies (miRVana protocol) collected from normal weight and obese individuals with periodontitis. MiRNA expression profiling was performed using Affymetrix GeneChip miRNA 3.0 arrays.

本研究针对肥胖与体重正常的牙周炎患者的牙龈组织活检样本，利用Affymetrix GeneChip miRNA 3.0微阵列（microarray）开展对比分析。实验整体设计：采用miRVana总RNA提取流程，从上述患者的牙龈组织活检样本中提取总RNA；随后使用Affymetrix GeneChip miRNA 3.0芯片完成微小RNA（microRNA，miRNA）的表达谱分析。