INDUSTRIAL PLANT FOR PRODUCTION OF Spirulina sp. LEB 18

ABSTRACT Biorefineries based on microalgae produce biofuels and co-products with added value. Microalgae mainly require water, carbon dioxide and sunlight for growth. The bioproducts of the cultivation of these microorganisms can be fully used in a microalgae photobiorefinery. The objective of this work was to study the behavior of physico-chemical variables and kinetic and biological responses in industrial cultivation of Spirulina sp. LEB 18, aiming at the operation of a microalgal photobiorefinery. The maximum specific growth rate (0.133 1/d), the minimum generation time (5.2 d) and maximum productivity (14.9 g/m².d) were obtained in the first 9 d of microalgae growth. The maximum biomass concentration (1.64 g/L) was obtained in 37 d of cultivation. The highest levels of carbohydrates, proteins and lipids in the biomass were 10.6, 57.0 and 11.7%, respectively. The plant monitoring demonstrated that the microalgae produced biomass with high quality for application as biofuels, energy, health and nutrition human.

摘要：基于微藻的生物炼制厂可产出生物燃料及高附加值联产品。微藻生长主要依赖水、二氧化碳与光照。此类微生物培养所得的生物产物可完全应用于微藻光生物炼制厂（photobiorefinery）流程中。本研究以微藻光生物炼制的工业化运行为目标，探究了螺旋藻（Spirulina）sp. LEB 18工业化培养过程中的理化变量变化、动力学及生物学响应。在微藻生长的前9天，测得最高比生长速率（0.133 d⁻¹）、最短代时（5.2 d）及最高生物质产率（14.9 g·m⁻²·d⁻¹）。培养37天时，获得最高生物质浓度（1.64 g/L）。生物质中碳水化合物、蛋白质与脂类的最高含量分别为10.6%、57.0%与11.7%。工厂监测结果表明，该微藻所产生物质品质优良，可应用于生物燃料、能源、健康及人类营养领域。