SH1403744.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE提供了一套统一的方法，用于界定、识别、交流以及处理基于DNA的物种假说（DNA-based Species Hypotheses，以下简称SH）。国际核酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，ITS）序列，通过应用一组动态距离阈值（0.5%至3.0%）被聚类为近似物种水平的单元。所有物种假说均被赋予以数字对象标识符（Digital Object Identifier，DOI）为格式的唯一稳定名称，其分类学与生态学注释均通过分布式的、基于网页的第三方注释工作完成核验。每个SH都会尽可能关联至对应的分类单元名称及其分类层级（如门、纲、目等），关联过程会综合考量该假说下所有序列的鉴定结果。研究人员会选取一条经自动或人工指定的序列，作为每个物种假说的代表序列。上述代表序列已发布于https://unite.ut.ee/repository.php，可供科研界用于本地序列相似性搜索、新一代测序分析流程等场景。随着公共真菌ITS序列数量的不断增长，该系统与配套数据集将自动进行更新。