Time course study of confinement stress in HR and LR trout lines. Oncorhynchus mykiss

Farmed fish are unavoidably exposed to husbandry-related stressors which can result in outbreaks of disease, reduced reproductive success and poor growth. Biomarkers are needed for the establishment of selective breeding approaches for the production of stress resistant fish. In this context, microarray analysis has been carried out on liver tissues of two genetic lines of rainbow trout, high responders (HR) and low responders (LR), after confinement for various time periods. Microarray hybridisations provided gene expression profiles for pools of fish (stress: n=5; control: n=3) after 6h, 24h and 168h of confinement stress. Keywords : stress response, pools, time course, comparison of two lines. Overall design: Samples were hybridised directly to each other in a parallel loop design. Each treatment (LC, LS, HC, HS) was hybidised in a loop of all 3 time points and each treatment loop was interconnected by hybridisations at a specific time. The publication will have this figure.

养殖鱼类不可避免会暴露于养殖相关应激源之下，进而引发疾病暴发、繁殖成功率下降与生长性能受损。当前亟需生物标志物（biomarker）以建立选育策略，培育抗应激养殖鱼类。在此背景下，研究针对两个遗传品系的虹鳟肝脏组织开展了微阵列（microarray）分析：两个品系分别为高应激响应组（High Responders, HR）与低应激响应组（Low Responders, LR），处理方式为施加不同时长的围捕应激。微阵列杂交实验获取了各鱼混合样本在围捕应激6小时、24小时及168小时后的基因表达谱（应激组：n=5；对照组：n=3）。关键词：应激响应、混合样本、时间进程、双品系比较。总体实验设计：样本采用平行环路设计进行两两直接杂交。每个处理组（LC、LS、HC、HS）均围绕全部三个时间点构建杂交环路，且各处理环路通过特定时间点的杂交实验实现互联。该研究论文将附带此实验图表。