Expression analysis of Reh cells after transfection with constitutively active variants of IRF5 (IRF5-4D) and/or constitutively active IKKβ(EE)

Genome-wide gene expression analysis of Reh cells following transfection with constitutively active IRF5-4D, constitutively active IKKβ(EE), or both in combination. Affymetrix U133 Plus 2.0 oligonucleotide arrays were hybridized to determine the gene expression profile of acute lymphoblastic leukemia-derived Reh cells following transfection with i) control constructs, ii), a constitutively active variant of IRF5 (IRF5-4D), iii) a constitutively active variant of the IkB kinase β (IKKβ(EE)), or iv) IRF5-4D in combination with IKKβ(EE). All hybridizations were done in biological duplicates.

本数据集针对转染组成型激活IRF5-4D、组成型激活IKKβ(EE)或二者联合转染的Reh细胞开展全基因组基因表达分析。实验采用Affymetrix U133 Plus 2.0寡核苷酸芯片进行杂交，以解析急性淋巴细胞白血病来源Reh细胞的基因表达谱，转染分组如下：i) 对照载体构建物，ii) 组成型激活型IRF5变体（IRF5-4D），iii) 组成型激活型IκB激酶β变体（IKKβ(EE)），iv) IRF5-4D与IKKβ(EE)联合转染组。所有杂交实验均完成生物学重复。