Transcriptome analysis of bovine cumulus cells during first 6 hrs of in vitro maturation. Bos taurus

Study the effect of in vitro maturation system on the transcriptomics of bovine cumulus cells during incubation of first 6 hrs. Overall design: 3 biological replicates of cumulus cells derived from cumulus oocyte complexes (COCs) after 6 hrs of in vitro matutaion(Ct-6H) vs. cumulus cells before maturation (Ct-0H, REFERENCE) were contrasted. Each replicate was subjected to a dye-swap procedure.

本研究旨在探究体外成熟系统（in vitro maturation system）对孵育前6小时内牛卵丘细胞转录组学（transcriptomics）的影响。
实验设计概述：将经6小时体外成熟培养的卵丘卵母细胞复合体（cumulus oocyte complexes, COCs）来源的卵丘细胞（Ct-6H）与成熟前卵丘细胞（Ct-0H，作为参照组）进行对比分析，共设置3个生物学重复，且每个重复均实施染料交换实验（dye-swap procedure）。