Comparison of Oryza sativa and Oryza officinalis Stigma and Pistil. Oryza officinalis

Comparison of the transcriptomes of stigma and pistil between Nipponbare and Oryza officinalis W0002. Samples are from pre-anthesis flowers and the stigma and pistils are prepared separately- a cut being made in the stylodium at the base of the stigma on each side. Pistil(not including stigmas) and stigmas 40 pre-anthesis flowers each of Oryza sativa ssp Japonica cv Nipponbare and Oryza officinalis W0002 were taken. Total RNA was prepared using Ambion RNAqueous micro RNA prep kit. 1ug total RNA was used as starting material and the Illumina mRNA seq sample preparation kit part # 1004898 Rev. A was used to prepare the library.

日本晴（Nipponbare）与药用野生稻（Oryza officinalis）W0002的柱头与雌蕊转录组比较分析。样本取自开花前小花，柱头与雌蕊采用分离制备：于两侧柱头基部的花柱处进行切割。分别采集粳稻（Oryza sativa ssp. Japonica）品种日本晴与药用野生稻W0002各40朵开花前小花的雌蕊（不含柱头）与柱头样本。总RNA提取采用Ambion RNAqueous微RNA制备试剂盒完成。以1μg总RNA作为起始原料，使用Illumina mRNA测序样本制备试剂盒（货号1004898 Rev. A）构建测序文库。