Differences in PBMC mRNA profiles between control and type 2 diabetic mice

Studies on immunometabolism have indicated that metabolism and the immunological state are inherently interconnected . Recent research has highlighted the impacts of metabolic syndrome on innate and adaptive immune function, which are linked to overall negative effects on the progression of related diseases . Notably, aging of immune system can be potentiated by dysmetabolism, culminating in chronic inflammation and immune fitness loss . Several age-related immune changes such as progressive loss of naive T cells, an increase in memory cells, and increased percentages of cells with immunosenescence markers, were identified in patients with T2DM . Dysfunction of both innate and adaptive immune responses in T2DM manifests similarly to aging, including poor control of infection and reduced vaccination response, along with elevated inflammatory activity.Hence, we sought to investigate the mechanism of accelerated immunosenescence during T2DM. To investigate the mechanisms involved in T2DM-induced immunosenescence, we isolated PBMCs from T2DM and age-matched control mice for RNA sequencing

免疫代谢学（immunometabolism）领域的研究表明，代谢状态与免疫状态本就存在内在关联。近期研究揭示了代谢综合征对固有免疫与适应性免疫功能的影响，这类影响与相关疾病进展的整体不良结局密切相关。值得注意的是，代谢异常可加剧免疫系统衰老，最终引发慢性炎症与免疫健康损失。在2型糖尿病（T2DM）患者体内，已发现多种与衰老相关的免疫改变：包括初始T细胞（naive T cells）进行性丢失、记忆细胞比例升高，以及携带免疫衰老标志物（immunosenescence markers）的细胞占比增加。2型糖尿病患者的固有免疫与适应性免疫应答功能障碍，其临床表现与衰老高度相似，具体包括感染控制能力下降、疫苗接种应答减弱，以及炎症活性升高。因此，本研究旨在探究2型糖尿病诱导免疫衰老加速的潜在机制。为阐明2型糖尿病介导免疫衰老的相关机制，我们从2型糖尿病小鼠及年龄匹配的对照小鼠体内分离外周血单个核细胞（Peripheral Blood Mononuclear Cells, PBMCs），并开展RNA测序（RNA sequencing）。