Protein EV contents from C. elegans

The hermaphrodite strain N2 (Bristol), fed with Escherichia coli OP50, was maintained and synchronized. Young adults were thoroughly washed before incubations for 5 h or 24 h in M9 buffer. Between 35,500 and 158,000 worms were used for each experiment. After incuabtion, supernatants were processed by differential centrigugation/ultracentrifugation to pellet extracellular vesicles and characterization of the protein contents by mass spectrometry.

以大肠杆菌（Escherichia coli）OP50饲喂的雌雄同体菌株N2（布里斯托尔株）经维持培养并同步化处理。在M9缓冲液中孵育5小时或24小时前，需对年轻成虫进行充分洗涤。每次实验使用35500至158000条线虫。孵育结束后，通过差速离心/超速离心处理上清液以沉淀细胞外囊泡，并采用质谱法对其蛋白质含量进行表征。