Chronic kidney disease and splicing events. Homo sapiens

Renal failure is associated with accumulation of various solutes called Uremic toxins. Post transcriptional regulation related to Chronic kidney disease (CKD) have already been described as RNA based silencing with micro RNA or modifications of mRNA degradation. Until now, alternative splice modification was not mentioned in the course of CKD. However, CKD is associated with modification of gene expression. The aim of the study was to explore modification of the alternative splice pattern in the course in CKD. Overall design: The expression level of individual exons expression in human fibroblast were compared after culture to 96 hours of uremic condition or control condition. Three independant experiments were performed

肾衰竭与被称为尿毒症毒素（Uremic toxins）的多种溶质蓄积密切相关。慢性肾脏病（Chronic kidney disease, CKD）相关的转录后调控此前已被描述为基于RNA的沉默机制（如微小RNA（micro RNA）调控）或mRNA降解修饰。截至目前，可变剪接修饰尚未在慢性肾脏病的病程中被提及。然而，慢性肾脏病与基因表达修饰存在关联。本研究旨在探索慢性肾脏病进程中可变剪接模式的修饰变化。整体实验设计：将人成纤维细胞（human fibroblast）分别置于尿毒症条件与对照条件下培养96小时后，对比二者的单个外显子（exons）表达水平。本研究共开展3次独立重复实验。